HPLC Column, Nova Similars Phenyl, 4um, 4.0mm ID x 250mm Length. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Nova Similars Phenyl™ is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped phenyl bonded phase. The stationary phase consists of a four-carbon linker terminating in a phenyl functional group, providing unique chromatographic selectivity through a combination of hydrophobic and π-π interactions. Designed as a cost-effective alternative to Waters™ Nova-Pak® Phenyl columns, it offers comparable chromatographic behavior for USP L11 methods while delivering the reproducibility and reliability required for routine analytical, quality control, and method-transfer applications.
64424-25P-4 is a 250 mm x 4.0 mm ID analytical column packed with 4 µm Nova Similars Phenyl particles. The extended 250 mm length provides increased theoretical plate counts and enhanced resolving power for impurity profiling, complex sample matrices, and challenging separations where maximum resolution is required. The 4 µm particle size provides improved efficiency and sharper peak shapes than comparable 5 µm columns while maintaining moderate operating pressure on conventional HPLC systems. The 4.0 mm ID format is commonly selected for legacy methods and typically operates at approximately 0.8–1.5 mL/min.
This USP L11 column is particularly useful for aromatic compounds where phenyl selectivity can provide different retention and separation characteristics than traditional C18 phases. Common applications include pharmaceutical analysis, aromatic hydrocarbons, environmental testing, impurity profiling, and methods requiring enhanced aromatic selectivity through π-π interactions. This product is supplied as a Special Order Item manufactured specifically upon receipt of order.
Key Benefits
Type-B spherical silica stationary phase
Suitable alternative for USP L11 methods
Comparable selectivity to Waters™ Nova-Pak® Phenyl columns
Fully endcapped phenyl bonded phase
Enhanced aromatic compound selectivity
Unique π-π interaction mechanism
Higher efficiency than comparable 5 µm columns
Increased resolution from 250 mm column length
Typical Applications
USP L11 methods
Reversed-phase HPLC
Pharmaceutical analysis
Aromatic compound separations
Environmental testing
Impurity profiling
Method development
Method transfer and validation
Specifications
Product Type: HPLC Column
Stationary Phase: Nova Similars Phenyl
USP Classification: L11
Particle Size: 4 µm
Particle Type: Type-B Spherical Silica
Bonded Phase: Phenyl
Endcapped: Fully
Column Dimensions: 4.0 mm ID x 250 mm
Length: 250 mm
Recommended Flow Rate: 0.8–1.5 mL/min
Brand: Cogent
Special Order Item Information
This item is a Special Order Item manufactured specifically to customer requirements.
Special Order Items cannot be canceled or returned once an order has been placed.
Manufactured upon receipt of order.
May have extended lead times compared to stocked products.
Important Notes
Designed for reversed-phase chromatography
Phenyl selectivity provides different retention than C18 phases
Enhanced interactions with aromatic compounds
Longer column length provides increased resolving power
Higher efficiency than comparable 5 µm columns
Moderate operating pressure on conventional HPLC systems
Special Order Item